US Ouagadougou 2-3 Aigle Royal *
| 04.04.2009 | Aigle Royal | 2-0 | US Ouagadougou | |
| 14.03.2009 | US Ouagadougou | 2-1 | Aigle Royal | |
APR FC 0-2 Haras Al Hodoud *
| 05.04.2009 | Haras Al Hodoud | 2-0 | APR FC | |
| 14.03.2009 | APR FC | 0-0 | Haras Al Hodoud | |
AS Vacoas-Phoenix 1-9 Mamelodi Sundowns *
| 05.04.2009 | Mamelodi Sundowns | 6-1 | AS Vacoas-Phoenix | |
| 15.03.2009 | AS Vacoas-Phoenix | 0-3 | Mamelodi Sundowns | |
Malanti Chiefs 1-6 AS Vita Club *
| 05.04.2009 | AS Vita Club | 3-0 | Malanti Chiefs | |
| 16.03.2009 | Malanti Chiefs | 1-3 | AS Vita Club | |
CAPS United FC 1-2 ENPPI Cairo *
| 03.04.2009 | ENPPI Cairo | 2-1 | CAPS United FC | |
| 15.03.2009 | CAPS United FC | 0-0 | ENPPI Cairo | |
Club 57 Tourbillon 0-1 Bayelsa United *
| 05.04.2009 | Bayelsa United | 1-0 | Club 57 Tourbillon | |
| 15.03.2009 | Club 57 Tourbillon | 0-0 | Bayelsa United | |
*CS Sfaxien 5-3 Al Ahli Benghazi
| 04.04.2009 | Al Ahli Benghazi | 2-1 | CS Sfaxien | |
| 14.03.2009 | CS Sfaxien | 4-1 | Al Ahli Benghazi | |
Maghreb Fès 1-2 EGS Gafsa *
| 05.04.2009 | EGS Gafsa | 1-0 | Maghreb Fès | |
| 14.03.2009 | Maghreb Fès | 1-1 | EGS Gafsa | |
Khaleej Sirte 0-6 ES Sétif *
| 03.04.2009 | ES Sétif | 5-0 | Khaleej Sirte | |
| 13.03.2009 | Khaleej Sirte | 0-1 | ES Sétif | |
Satellite FC 2-5 JC Abidjan *
| 05.04.2009 | JC Abidjan | 3-1 | Satellite FC | |
| 15.03.2009 | Satellite FC | 1-2 | JC Abidjan | |
ASC Yakaar 2-5 JSM Béjaia *
| 03.04.2009 | JSM Béjaia | 4-1 | ASC Yakaar | |
| 15.03.2009 | ASC Yakaar | 1-1 | JSM Béjaia | |
SO Armée 3-6 Recreativo Libolo *
| 04.04.2009 | Recreativo Libolo | 5-3 | SO Armée | |
| 13.03.2009 | SO Armée | 0-1 | Recreativo Libolo | |
*Red Arrows FC 2-2 Ocean Boys
| 04.04.2009 | Ocean Boys | 2-0/p.3-5 | Red Arrows FC | |
| 14.03.2009 | Red Arrows FC | 2-0 | Ocean Boys | |
USM Libreville 2-5 Santos de Angola *
| 05.04.2009 | Santos de Angola | 4-2 | USM Libreville | |
| 15.03.2009 | USM Libreville | 0-1 | Santos de Angola | |
Stade Tunisien 0-2 Stade Malien *
| 04.04.2009 | Stade Malien | 2-0 | Stade Tunisien | |
| 15.03.2009 | Stade Tunisien | 0-0 | Stade Malien | |
Tourbillon FC 3-5 Union Douala *
| 05.04.2009 | Union Douala | 3-2 | Tourbillon FC | |
| 14.03.2009 | Tourbillon FC | 1-2 | Union Douala | |