Mamelodi Sundowns 2-3 AS Vita Club *
| 03.05.2009 | AS Vita Club | 1-0 | Mamelodi Sundowns | |
| 18.04.2009 | Mamelodi Sundowns | 2-2 | AS Vita Club | |
Aigle Royal 1-5 Bayelsa United *
| 03.05.2009 | Bayelsa United | 5-0 | Aigle Royal | |
| 18.04.2009 | Aigle Royal | 1-0 | Bayelsa United | |
*CS Sfaxien 3-0 JC Abidjan
| 03.05.2009 | JC Abidjan | 0-1 | CS Sfaxien | |
| 19.04.2009 | CS Sfaxien | 2-0 | JC Abidjan | |
*ENPPI Cairo 4-3 Red Arrows FC
| 02.05.2009 | Red Arrows FC | 3-0 | ENPPI Cairo | |
| 18.04.2009 | ENPPI Cairo | 4-0 | Red Arrows FC | |
Haras Al Hodoud 6-1 EGS Gafsa
| 03.05.2009 | EGS Gafsa | 1-3 | Haras Al Hodoud | |
| 19.04.2009 | Haras Al Hodoud | 3-0 | EGS Gafsa | |
JSM Béjaia 1-1 Stade Malien *
| 02.05.2009 | Stade Malien | 1-0/p.13-12 | JSM Béjaia | |
| 18.04.2009 | JSM Béjaia | 1-0 | Stade Malien | |
*ES Sétif 5-5 Recreativo Libolo
| 03.05.2009 | Recreativo Libolo | 5-1 | ES Sétif | |
| 17.04.2009 | ES Sétif | 4-0 | Recreativo Libolo | |
Union Douala 1-5 Santos de Angola *
| 03.05.2009 | Santos de Angola | 4-0 | Union Douala | |
| 19.04.2009 | Union Douala | 1-1 | Santos de Angola | |