ABC Natal/RN 3-5 Atlético/PR *
| 23.04.2009 | Atlético/PR | 3-1 | ABC Natal/RN | |
| 09.04.2009 | ABC Natal/RN | 2-2 | Atlético/PR | |
Águia de Marabá/PA 2-4 Fluminense/RJ *
| 23.04.2009 | Fluminense/RJ | 3-0 | Águia de Marabá/PA | |
| 17.04.2009 | Águia de Marabá/PA | 2-1 | Fluminense/RJ | |
Bahia/BA 2-2 Coritiba/PR *
| 16.04.2009 | Coritiba/PR | 0-0 | Bahia/BA | |
| 09.04.2009 | Bahia/BA | 2-2 | Coritiba/PR | |
*Americano/RJ 3-3 Botafogo/RJ
| 17.04.2009 | Botafogo/RJ | 2-1/p.4-5 | Americano/RJ | |
| 09.04.2009 | Americano/RJ | 2-1 | Botafogo/RJ | |
Central/PE 0-3 Vasco da Gama/RJ *
| 16.04.2009 | Central/PE | 0-3 | Vasco da Gama/RJ | |
*Icasa/CE 2-1 Confiança/SE
| 23.04.2009 | Confiança/SE | 1-2 | Icasa/CE | |
| 16.04.2009 | Icasa/CE | 0-0 | Confiança/SE | |
Criciúma/SC 4-5 Nautico/PE *
| 24.04.2009 | Nautico/PE | 3-2 | Criciúma/SC | |
| 16.04.2009 | Criciúma/SC | 2-2 | Nautico/PE | |
Brasiliense/DF 1-5 Goiás/GO *
| 17.04.2009 | Goiás/GO | 4-1 | Brasiliense/DF | |
| 09.04.2009 | Brasiliense/DF | 0-1 | Goiás/GO | |
Guarani/SP 1-7 Internacional/RS *
| 23.04.2009 | Internacional/RS | 5-0 | Guarani/SP | |
| 09.04.2009 | Guarani/SP | 1-2 | Internacional/RS | |
Guaratinguetá/SP 2-4 Atlético Mineiro/MG *
| 24.04.2009 | Atlético Mineiro/MG | 2-0 | Guaratinguetá/SP | |
| 16.04.2009 | Guaratinguetá/SP | 2-2 | Atlético Mineiro/MG | |
Misto/MS 0-2 Corinthians/SP *
| 16.04.2009 | Misto/MS | 0-2 | Corinthians/SP | |
*Fortaleza/CE 3-2 Paraná/PR
| 23.04.2009 | Paraná/PR | 1-1 | Fortaleza/CE | |
| 16.04.2009 | Fortaleza/CE | 2-1 | Paraná/PR | |
Figueirense/SC 2-2 Ponte Preta/SP *
| 23.04.2009 | Ponte Preta/SP | 0-0 | Figueirense/SC | |
| 10.04.2009 | Figueirense/SC | 2-2 | Ponte Preta/SP | |
Remo/PA 0-2 Flamengo/RJ *
| 09.04.2009 | Remo/PA | 0-2 | Flamengo/RJ | |
Juventude/RS 2-3 Vitória/BA *
| 16.04.2009 | Vitória/BA | 1-1 | Juventude/RS | |
| 09.04.2009 | Juventude/RS | 1-2 | Vitória/BA | |