Alecrim Natal/RN 2-1 Central/PE
| 23.08.2009 | Central/PE | 1-0 | Alecrim Natal/RN | |
| 16.08.2009 | Alecrim Natal/RN | 2-0 | Central/PE | |
Corinthians/PR 0-3 Chapecoense/SC
| 23.08.2009 | Chapecoense/SC | 0-0 | Corinthians/PR | |
| 16.08.2009 | Corinthians/PR | 0-3 | Chapecoense/SC | |
Cristal/AP 6-3 Nacional/AM
| 23.08.2009 | Nacional/AM | 2-5 | Cristal/AP | |
| 16.08.2009 | Cristal/AP | 1-1 | Nacional/AM | |
Sergipe/SE 5-3 Ferroviário/CE
| 23.08.2009 | Ferroviário/CE | 3-3 | Sergipe/SE | |
| 16.08.2009 | Sergipe/SE | 2-0 | Ferroviário/CE | |
Fluminense Feira/BA 3-5 Tupi/MG *
| 22.08.2009 | Tupi/MG | 4-2 | Fluminense Feira/BA | |
| 19.08.2009 | Fluminense Feira/BA | 1-1 | Tupi/MG | |
Londrina/PR 3-2 São José PoA/RS
| 22.08.2009 | São José PoA/RS | 1-0 | Londrina/PR | |
| 16.08.2009 | Londrina/PR | 3-1 | São José PoA/RS | |
Paulista/SP 1-3 Macaé/RJ
| 23.08.2009 | Macaé/RJ | 3-1 | Paulista/SP | |
| 16.08.2009 | Paulista/SP | 0-0 | Macaé/RJ | |
Genus/RO 1-3 São Raimundo/PA
| 23.08.2009 | São Raimundo/PA | 2-0 | Genus/RO | |
| 17.08.2009 | Genus/RO | 1-1 | São Raimundo/PA | |
Brasília FC/DF 3-4 Uberaba/MG *
| 22.08.2009 | Uberaba/MG | 2-0 | Brasília FC/DF | |
| 15.08.2009 | Brasília FC/DF | 3-2 | Uberaba/MG | |
Uberlândia/MG 3-4 Araguaia/MT
| 23.08.2009 | Araguaia/MT | 2-0 | Uberlândia/MG | |
| 19.08.2009 | Uberlândia/MG | 3-2 | Araguaia/MT | |