FK Teplice 2-0 Slovan Liberec
| 22.04.2009 | Slovan Liberec | 0-2 | FK Teplice | |
| 09.04.2009 | FK Teplice | 0-0 | Slovan Liberec | |
FK Zlin 0-3 Slavia Praha
| 23.04.2009 | Slavia Praha | 3-0 | FK Zlin | |
| 08.04.2009 | FK Zlin | 0-0 | Slavia Praha | |
Slovacko 2-1 Banik Ostrava
| 22.04.2009 | Banik Ostrava | 1-1 | Slovacko | |
| 08.04.2009 | Slovacko | 1-0 | Banik Ostrava | |
Sparta Praha 3-0 Viktoria Plzen
| 22.04.2009 | Viktoria Plzen | 0-3 | Sparta Praha | |
| 09.04.2009 | Sparta Praha | 0-0 | Viktoria Plzen | |