*Slavia Praha 2-0 Sparta Praha
| 15.04.2010 | Sparta Praha | 0-1 | Slavia Praha | |
| 01.04.2010 | Slavia Praha | 1-0 | Sparta Praha | |
Slovan Liberec 0-3 Sigma Olomouc
| 07.04.2010 | Sigma Olomouc | 1-0 | Slovan Liberec | |
| 31.03.2010 | Slovan Liberec | 0-2 | Sigma Olomouc | |
Teplice 1-3 FK Jablonec *
| 08.04.2010 | FK Jablonec | 0-0 | Teplice | |
| 31.03.2010 | Teplice | 1-3 | FK Jablonec | |
*Viktoria Plzen 2-1 Pribram
| 08.04.2010 | Pribram | 1-1 | Viktoria Plzen | |
| 31.03.2010 | Viktoria Plzen | 1-0 | Pribram | |