MFK OKD Karvina 2-4 1. FC Brno *
| 10.11.2010 | 1. FC Brno | 4-0 | MFK OKD Karvina | |
| 27.10.2010 | MFK OKD Karvina | 2-0 | 1. FC Brno | |
*Teplice 2-1 Ceske Budejovice
| 10.11.2010 | Ceske Budejovice | 0-2 | Teplice | |
| 03.11.2010 | Teplice | 0-1 | Ceske Budejovice | |
FC Pisek 4-4 Sigma Olomouc *
| 10.11.2010 | Sigma Olomouc | 1-1 | FC Pisek | |
| 27.10.2010 | FC Pisek | 3-3 | Sigma Olomouc | |
HFK Olomouc 4-7 FK Jablonec *
| 10.11.2010 | FK Jablonec | 5-0 | HFK Olomouc | |
| 27.10.2010 | HFK Olomouc | 4-2 | FK Jablonec | |
Banik Sokolov 3-3 Mlada Boleslav *
| 10.11.2010 | Mlada Boleslav | 3-0/p.6-5 | Banik Sokolov | |
| 27.10.2010 | Banik Sokolov | 3-0 | Mlada Boleslav | |
Slavicin 1-5 H. Slavia Kromeriz
| 17.11.2010 | H. Slavia Kromeriz | 0-0 | Slavicin | |
| 27.10.2010 | Slavicin | 1-5 | H. Slavia Kromeriz | |
Viktoria Plzen 6-3 Opava
| 23.11.2010 | Opava | 3-3 | Viktoria Plzen | |
| 10.11.2010 | Viktoria Plzen | 3-0 | Opava | |
Viktoria Zizkov 1-4 Slavia Praha
| 09.11.2010 | Slavia Praha | 3-0 | Viktoria Zizkov | |
| 27.10.2010 | Viktoria Zizkov | 1-1 | Slavia Praha | |