| 25.08.2012 | |||
| 16:00 | Bonnyrigg Rose | 2-1 | Girvan FC |
| 16:00 | Buckie Thistle | 0-0 | Rothes FC |
| 16:00 | Civil Service Strollers | 4-0 | Newton Stewart |
| 16:00 | Clachnacuddin | 2-1 | Lossiemouth FC |
| 16:00 | Edinburgh Univ AFC | 1-2 | St. Cuthbert Wanderers |
| 16:00 | Formartine United | 3-2 | Brora Rangers |
| 16:00 | Fraserburgh FC | 4-0 | Coldstream FC |
| 16:00 | Glasgow University | 0-2 | Selkirk FC |
| 16:00 | Hawick Royal Albert | 1-4 | Golspie Sutherland |
| 16:00 | Hermes Junior FC | 1-4 | Deveronvale FC |
| 16:00 | Huntly FC | 2-2 | Wigtown & Bladnoch |
| 16:00 | Irvine Meadow XI FC | 4-0 | Gala Fairydean |
| 16:00 | Preston Athletic | 0-2 | Nairn County |
| 16:00 | Shotts Bon Accord | 1-1 | Edinburgh City |
| 16:00 | Spartans FC | 0-2 | Wick Academy |
| 16:00 | Threave Rovers | 0-1 | Vale of Leithen |
| 16:00 | Turriff United | 6-1 | Burntisland Shipyard AFC |
| 16:00 | Whitehill Welfare | 2-4 | Inverurie Loco Works |
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