| 11.09.2008 | |||
| 12:00 | Gavà CF | 1-3 AET | Universidad Las Palmas |
| 04.09.2008 | |||
| 20:30 | Alicante CF | 0-1 | Celta de Vigo |
| 03.09.2008 | |||
| 22:00 | CD Tenerife | 2-1 | Córdoba CF |
| 21:50 | UD Melilla | 5-2 | Mérida UD |
| 21:30 | Écija Balompié | 0-1 | Club Portugalete |
| 21:30 | Hércules CF | 2-1 AET | Levante UD |
| 21:30 | Polideportivo Ejido | 2-0 | UB Conquense |
| 21:00 | Benidorm CF* | 4-2 AET | SD Lemona |
| 21:00 | CD Alfaro | 1-2 | CD Don Benito |
| 21:00 | CD Castellón | 1-0 | SD Eibar |
| 21:00 | Elche CF | 2-0 | Deportivo Alavés |
| 21:00 | Gimnàstic Tarragona | 0-2 | Girona FC |
| 21:00 | UD Alzira | 0-0/p.1-4 | Granada 74 |
| 21:00 | Xerez CD | 1-1/p.1-3 | Real Murcia |
| 20:45 | UD Salamanca | 2-1 | UD Las Palmas |
| 20:30 | Barakaldo CF | 1-0 AET | CD Roquetas |
| 20:30 | CD Toledo | 1-0 | Zamora CF |
| 20:30 | Orihuela CF | 1-0 | UD Fuerteventura |
| 20:20 | SD Huesca | 1-3 AET | Rayo Vallecano |
| 20:15 | Real Oviedo | 2-3 AET | SD Ponferradina |
| 20:15 | Real Unión | 2-1 | UE Sant Andreu |
| 20:00 | Real Sociedad | 1-0 | Real Zaragoza |
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