Colorado Rapids 2-4 Kansas City Wizards *
| 24.09.2000 | Colorado Rapids | 2-3 | Kansas City Wizards | |
| 20.09.2000 | Kansas City Wizards | 0-0 | Colorado Rapids | |
| 16.09.2000 | Colorado Rapids | 0-1 | Kansas City Wizards | |
*Los Angeles Galaxy 6-2 Tampa Bay Mutiny
| 20.09.2000 | Tampa Bay Mutiny | 2-5 | Los Angeles Galaxy | |
| 14.09.2000 | Los Angeles Galaxy | 1-0 | Tampa Bay Mutiny | |
New England Revs 3-9 Chicago Fire *
| 22.09.2000 | New England Revs | 0-6 | Chicago Fire | |
| 19.09.2000 | Chicago Fire | 1-2 | New England Revs | |
| 15.09.2000 | New England Revs | 1-2 | Chicago Fire | |
Dallas Burn 2-4 NY/NJ MetroStars *
| 20.09.2000 | NY/NJ MetroStars | 2-1 | Dallas Burn | |
| 15.09.2000 | Dallas Burn | 1-2 AET | NY/NJ MetroStars | |