Africa Sports 0-2 ZESCO United *
| 04.04.2009 | ZESCO United | 2-0 | Africa Sports | |
| 15.03.2009 | Africa Sports | 0-0 | ZESCO United | |
Ajax Cape Town 4-4 Monomotapa United *
| 05.04.2009 | Monomotapa United | 2-1 | Ajax Cape Town | |
| 14.03.2009 | Ajax Cape Town | 3-2 | Monomotapa United | |
JS Kabylie 1-3 Al Ahli Tripoli *
| 04.04.2009 | Al Ahli Tripoli | 1-0 | JS Kabylie | |
| 15.03.2009 | JS Kabylie | 1-2 | Al Ahli Tripoli | |
*Al Hilal Al Sudani 4-3 US Stade Tamponnaise
| 05.04.2009 | US Stade Tamponnaise | 2-1 | Al Hilal Al Sudani | |
| 15.03.2009 | Al Hilal Al Sudani | 3-1 | US Stade Tamponnaise | |
AS Douanes Dakar 1-1 Kano Pillars *
| 04.04.2009 | Kano Pillars | 0-0 | AS Douanes Dakar | |
| 14.03.2009 | AS Douanes Dakar | 1-1 | Kano Pillars | |
Asante Kotoko 3-3 Ittihad Khémisset *
| 05.04.2009 | Ittihad Khémisset | 2-0 | Asante Kotoko | |
| 15.03.2009 | Asante Kotoko | 3-1 | Ittihad Khémisset | |
*Étoile Sahel Sousse 2-1 ASO Chlef
| 03.04.2009 | ASO Chlef | 0-0 | Étoile Sahel Sousse | |
| 14.03.2009 | Étoile Sahel Sousse | 2-1 | ASO Chlef | |
*1º de Agosto 1-1 Canon Yaoundé
| 06.04.2009 | Canon Yaoundé | 0-1/p.4-5 | 1º de Agosto | |
| 14.03.2009 | 1º de Agosto | 0-1 | Canon Yaoundé | |
*Coton Sport 5-3 AS Mangasport
| 04.04.2009 | AS Mangasport | 2-3 | Coton Sport | |
| 15.03.2009 | Coton Sport | 2-1 | AS Mangasport | |
Club Africain Tunis 2-2 Djoliba AC *
| 05.04.2009 | Djoliba AC | 0-1 | Club Africain Tunis | |
| 15.03.2009 | Club Africain Tunis | 1-2 | Djoliba AC | |
*ASEC Mimosas 3-0 Étoile Filante
| 05.04.2009 | Étoile Filante | 0-1 | ASEC Mimosas | |
| 14.03.2009 | ASEC Mimosas | 2-0 | Étoile Filante | |
*Heartland FC 4-2 FAR Rabat
| 04.04.2009 | FAR Rabat | 1-1 | Heartland FC | |
| 15.03.2009 | Heartland FC | 3-1 | FAR Rabat | |
Ittihad Tripoli 1-4 Merreikh Al Sudan *
| 04.04.2009 | Merreikh Al Sudan | 3-0 | Ittihad Tripoli | |
| 13.03.2009 | Ittihad Tripoli | 1-1 | Merreikh Al Sudan | |
*KCC Kampala 3-2 SuperSport United
| 04.04.2009 | SuperSport United | 1-1 | KCC Kampala | |
| 13.03.2009 | KCC Kampala | 2-1 | SuperSport United | |
*TP Mazembe 5-1 Petro Atlético Luanda
| 04.04.2009 | Petro Atlético Luanda | 1-2 | TP Mazembe | |
| 15.03.2009 | TP Mazembe | 3-0 | Petro Atlético Luanda | |
*Ahly Cairo 4-0 Young Africans
| 04.04.2009 | Young Africans | 0-1 | Ahly Cairo | |
| 15.03.2009 | Ahly Cairo | 3-0 | Young Africans | |