US Stade Tamponnaise 5-2 Ajesaia Antananarivo
| 28.02.2010 | Ajesaia Antananarivo | 1-3 | US Stade Tamponnaise | |
| 13.02.2010 | US Stade Tamponnaise | 2-1 | Ajesaia Antananarivo | |
Apaches Club 4-9 Ferroviário Maputo
| 27.02.2010 | Ferroviário Maputo | 4-1 | Apaches Club | |
| 13.02.2010 | Apaches Club | 3-5 | Ferroviário Maputo | |
APR FC 3-2 Recreativo Libolo
| 28.02.2010 | Recreativo Libolo | 0-1 | APR FC | |
| 13.02.2010 | APR FC | 2-2 | Recreativo Libolo | |
Armed Forces FC 1-5 JS Kabylie *
| 27.02.2010 | JS Kabylie | 3-0 | Armed Forces FC | |
| 13.02.2010 | Armed Forces FC | 1-2 | JS Kabylie | |
ASC Linguère 2-2 Asante Kotoko
| 28.02.2010 | Asante Kotoko | 2-0/p.2-4 | ASC Linguère | |
| 13.02.2010 | ASC Linguère | 2-0 | Asante Kotoko | |
AS Stade Mandji 1-6 ASFA Yennega
| 28.02.2010 | ASFA Yennega | 4-1 | AS Stade Mandji | |
| 14.02.2010 | AS Stade Mandji | 0-2 | ASFA Yennega | |
Sahel SC Niamey 2-2 Club Africain Tunis *
| 26.02.2010 | Club Africain Tunis | 1-0 | Sahel SC Niamey | |
| 14.02.2010 | Sahel SC Niamey | 2-1 | Club Africain Tunis | |
La Passe FC 2-3 Curepipe Starlight
| 28.02.2010 | Curepipe Starlight | 3-1 | La Passe FC | |
| 13.02.2010 | La Passe FC | 1-0 | Curepipe Starlight | |
Os Balantas Mansôa 0-3 Difaâ El Jadidi
| 27.02.2010 | Difaâ El Jadidi | 3-0 | Os Balantas Mansôa | |
| 13.02.2010 | Os Balantas Mansôa | 0-0 | Difaâ El Jadidi | |
*Djoliba AC 1-0 Al Ahli Benghazi
| 28.02.2010 | Al Ahli Benghazi | 0-0 | Djoliba AC | |
| 14.02.2010 | Djoliba AC | 1-0 | Al Ahli Benghazi | |
East End Lions FC 4-5 Esperance ST
| 26.02.2010 | Esperance ST | 3-2 | East End Lions FC | |
| 13.02.2010 | East End Lions FC | 2-2 | Esperance ST | |
Diables Noirs 3-4 ES Sétif
| 27.02.2010 | ES Sétif | 2-0 | Diables Noirs | |
| 14.02.2010 | Diables Noirs | 3-2 | ES Sétif | |
Fello Star 2-4 Raja Casablanca *
| 28.02.2010 | Raja Casablanca | 1-1 | Fello Star | |
| 14.02.2010 | Fello Star | 1-3 | Raja Casablanca | |
Sofapaka Nairobi 0-2 Ismaily SC
| 27.02.2010 | Ismaily SC | 2-0 | Sofapaka Nairobi | |
| 13.02.2010 | Sofapaka Nairobi | 0-0 | Ismaily SC | |
AS Tempête Mocaf 1-8 Ittihad Tripoli
| 27.02.2010 | Ittihad Tripoli | 6-0 | AS Tempête Mocaf | |
| 14.02.2010 | AS Tempête Mocaf | 1-2 | Ittihad Tripoli | |
Mafunzo FC 1-6 Gunners FC
| 28.02.2010 | Gunners FC | 4-0 | Mafunzo FC | |
| 14.02.2010 | Mafunzo FC | 1-2 | Gunners FC | |
Mbabane Swallows 3-5 SuperSport United
| 27.02.2010 | SuperSport United | 2-2 | Mbabane Swallows | |
| 14.02.2010 | Mbabane Swallows | 1-3 | SuperSport United | |
Gaborone United 2-2 Orlando Pirates
| 28.02.2010 | Orlando Pirates | 2-2 | Gaborone United | |
| 13.02.2010 | Gaborone United | 0-0 | Orlando Pirates | |
CD Elá Nguema 3-9 Petro Atlético Luanda
| 28.02.2010 | Petro Atlético Luanda | 6-1 | CD Elá Nguema | |
| 14.02.2010 | CD Elá Nguema | 2-3 | Petro Atlético Luanda | |
Simba SC 2-4 Saint Eloi Lupopo
| 27.02.2010 | Saint Eloi Lupopo | 1-0 | Simba SC | |
| 13.02.2010 | Simba SC | 2-3 | Saint Eloi Lupopo | |
Saint George SA 2-4 Merreikh Al Sudan
| 27.02.2010 | Merreikh Al Sudan | 3-1 | Saint George SA | |
| 14.02.2010 | Saint George SA | 1-1 | Merreikh Al Sudan | |
Vital'O FC 4-4 Tiko United
| 27.02.2010 | Tiko United | 2-2/p.5-4 | Vital'O FC | |
| 14.02.2010 | Vital'O FC | 2-2 | Tiko United | |