Africa Sports 1-4 Al Hilal Al Sudani *
| 04.04.2010 | Al Hilal Al Sudani | 4-1 | Africa Sports | |
| 21.03.2010 | Africa Sports | 0-0 | Al Hilal Al Sudani | |
Djoliba AC 1-1 ASC Linguère
| 05.04.2010 | ASC Linguère | 1-0/p.3-4 | Djoliba AC | |
| 21.03.2010 | Djoliba AC | 1-0 | ASC Linguère | |
Club Africain Tunis 1-2 JS Kabylie *
| 02.04.2010 | JS Kabylie | 1-0 | Club Africain Tunis | |
| 19.03.2010 | Club Africain Tunis | 1-1 | JS Kabylie | |
*Ittihad Tripoli 2-2 Difaâ El Jadidi
| 02.04.2010 | Difaâ El Jadidi | 1-1/p.3-4 | Ittihad Tripoli | |
| 19.03.2010 | Ittihad Tripoli | 1-1 | Difaâ El Jadidi | |
Union Douala 0-7 ES Sétif *
| 02.04.2010 | ES Sétif | 5-0 | Union Douala | |
| 21.03.2010 | Union Douala | 0-2 | ES Sétif | |
*Esperance ST 7-2 ASFA Yennega
| 03.04.2010 | ASFA Yennega | 1-3 | Esperance ST | |
| 20.03.2010 | Esperance ST | 4-1 | ASFA Yennega | |
*SuperSport United 3-2 Ferroviário Maputo
| 03.04.2010 | Ferroviário Maputo | 2-0 | SuperSport United | |
| 20.03.2010 | SuperSport United | 3-0 | Ferroviário Maputo | |
Curepipe Starlight 0-6 Gaborone United *
| 02.04.2010 | Gaborone United | 3-0 | Curepipe Starlight | |
| 20.03.2010 | Curepipe Starlight | 0-3 | Gaborone United | |
Gazelle N'Djamena 1-3 Al Merreikh SC *
| 03.04.2010 | Al Merreikh SC | 2-0 | Gazelle N'Djamena | |
| 21.03.2010 | Gazelle N'Djamena | 1-1 | Al Merreikh SC | |
Gunners FC 1-2 Al Ahly Cairo *
| 02.04.2010 | Al Ahly Cairo | 2-0 | Gunners FC | |
| 20.03.2010 | Gunners FC | 1-0 | Al Ahly Cairo | |
Tiko United 3-3 Heartland FC *
| 04.04.2010 | Heartland FC | 1-1 | Tiko United | |
| 20.03.2010 | Tiko United | 2-2 | Heartland FC | |
*Ismaily SC 3-2 US Stade Tamponnaise
| 03.04.2010 | US Stade Tamponnaise | 1-0 | Ismaily SC | |
| 20.03.2010 | Ismaily SC | 3-1 | US Stade Tamponnaise | |
Raja Casablanca 1-2 Petro Atlético Luanda
| 04.04.2010 | Petro Atlético Luanda | 1-0 | Raja Casablanca | |
| 21.03.2010 | Raja Casablanca | 1-1 | Petro Atlético Luanda | |
Saint Eloi Lupopo 0-2 Dynamos FC *
| 04.04.2010 | Dynamos FC | 1-0 | Saint Eloi Lupopo | |
| 21.03.2010 | Saint Eloi Lupopo | 0-1 | Dynamos FC | |
APR FC 1-2 TP Mazembe *
| 04.04.2010 | TP Mazembe | 2-0 | APR FC | |
| 20.03.2010 | APR FC | 1-0 | TP Mazembe | |
*Zanaco FC 2-1 ASEC Mimosas
| 04.04.2010 | ASEC Mimosas | 1-1 | Zanaco FC | |
| 20.03.2010 | Zanaco FC | 1-0 | ASEC Mimosas | |