Cercle Olympique 0-3 1º de Agosto *
| 03.04.2010 | 1º de Agosto | 3-0 | Cercle Olympique | |
| 20.03.2010 | Cercle Olympique | 0-0 | 1º de Agosto | |
AC Léopard 3-3 Coton Sport *
| 03.04.2010 | Coton Sport | 2-0 | AC Léopard | |
| 21.03.2010 | AC Léopard | 3-1 | Coton Sport | |
Al Ahli Tripoli 0-1 CS Sfaxien *
| 02.04.2010 | CS Sfaxien | 1-0 | Al Ahli Tripoli | |
| 20.03.2010 | Al Ahli Tripoli | 0-0 | CS Sfaxien | |
*Al Amal Atbara 6-5 Costa do Sol Maputo
| 04.04.2010 | Costa do Sol Maputo | 3-2 | Al Amal Atbara | |
| 21.03.2010 | Al Amal Atbara | 4-2 | Costa do Sol Maputo | |
AS Baraka Djoma 0-1 FUS Rabat *
| 02.04.2010 | FUS Rabat | 1-0 | AS Baraka Djoma | |
| 21.03.2010 | AS Baraka Djoma | 0-0 | FUS Rabat | |
*CR Belouizdad 2-1 FAR Rabat
| 03.04.2010 | FAR Rabat | 1-1 | CR Belouizdad | |
| 20.03.2010 | CR Belouizdad | 1-0 | FAR Rabat | |
*DC Motema Pembe 1-0 FC 105 Libreville
| 03.04.2010 | FC 105 Libreville | 0-1 | DC Motema Pembe | |
| 20.03.2010 | DC Motema Pembe | 0-0 | FC 105 Libreville | |
Académica Lobito 2-3 Enyimba FC *
| 04.04.2010 | Enyimba FC | 3-0 | Académica Lobito | |
| 20.03.2010 | Académica Lobito | 2-0 | Enyimba FC | |
*ASFAN Niamey 2-2 Étoile Sahel Sousse
| 02.04.2010 | Étoile Sahel Sousse | 2-1 | ASFAN Niamey | |
| 21.03.2010 | ASFAN Niamey | 1-0 | Étoile Sahel Sousse | |
Banks SC 1-6 Haras Al Hodoud *
| 03.04.2010 | Haras Al Hodoud | 5-0 | Banks SC | |
| 21.03.2010 | Banks SC | 1-1 | Haras Al Hodoud | |
Lengthens FC 1-5 Simba SC *
| 04.04.2010 | Simba SC | 2-1 | Lengthens FC | |
| 19.03.2010 | Lengthens FC | 0-3 | Simba SC | |
*CAPS United FC 2-1 Moroka Swallows
| 03.04.2010 | Moroka Swallows | 0-1 | CAPS United FC | |
| 19.03.2010 | CAPS United FC | 1-1 | Moroka Swallows | |
Panthère du Ndé 3-4 AS Vita Club *
| 03.04.2010 | AS Vita Club | 3-2 | Panthère du Ndé | |
| 21.03.2010 | Panthère du Ndé | 1-1 | AS Vita Club | |
*Petrojet Suez 5-1 Khartoum Al Watani
| 02.04.2010 | Khartoum Al Watani | 1-2 | Petrojet Suez | |
| 21.03.2010 | Petrojet Suez | 3-0 | Khartoum Al Watani | |
Séwé Sports 2-2 Stade Malien *
| 03.04.2010 | Stade Malien | 2-0/p.4-3 | Séwé Sports | |
| 20.03.2010 | Séwé Sports | 2-0 | Stade Malien | |
*Warri Wolves 3-2 ZESCO United
| 03.04.2010 | ZESCO United | 2-0 | Warri Wolves | |
| 21.03.2010 | Warri Wolves | 3-0 | ZESCO United | |