Athletic Bilbao 1-5 Anderlecht *
| 25.02.2010 | Anderlecht | 4-0 | Athletic Bilbao | |
| 18.02.2010 | Athletic Bilbao | 1-1 | Anderlecht | |
FC København 2-6 Olympique Marseille *
| 25.02.2010 | Olympique Marseille | 3-1 | FC København | |
| 18.02.2010 | FC København | 1-3 | Olympique Marseille | |
*Atlético Madrid 3-2 Galatasaray SK
| 25.02.2010 | Galatasaray SK | 1-2 | Atlético Madrid | |
| 18.02.2010 | Atlético Madrid | 1-1 | Galatasaray SK | |
*Hamburger SV 3-3 PSV Eindhoven
| 25.02.2010 | PSV Eindhoven | 3-2 | Hamburger SV | |
| 18.02.2010 | Hamburger SV | 1-0 | PSV Eindhoven | |
AFC Ajax 1-2 Juventus FC *
| 25.02.2010 | Juventus FC | 0-0 | AFC Ajax | |
| 18.02.2010 | AFC Ajax | 1-2 | Juventus FC | |
*Lille OSC 3-2 Fenerbahçe SK
| 25.02.2010 | Fenerbahçe SK | 1-1 | Lille OSC | |
| 18.02.2010 | Lille OSC | 2-1 | Fenerbahçe SK | |
*Panathinaikos 6-4 AS Roma
| 25.02.2010 | AS Roma | 2-3 | Panathinaikos | |
| 18.02.2010 | Panathinaikos | 3-2 | AS Roma | |
*Standard Liège 3-2 RB Salzburg
| 25.02.2010 | RB Salzburg | 0-0 | Standard Liège | |
| 18.02.2010 | Standard Liège | 3-2 | RB Salzburg | |
*Rubin Kazan 3-0 Hapoel Tel Aviv
| 25.02.2010 | Hapoel Tel Aviv | 0-0 | Rubin Kazan | |
| 18.02.2010 | Rubin Kazan | 3-0 | Hapoel Tel Aviv | |
*Fulham 3-2 Shakhtar Donetsk
| 25.02.2010 | Shakhtar Donetsk | 1-1 | Fulham | |
| 18.02.2010 | Fulham | 2-1 | Shakhtar Donetsk | |
Hertha BSC 1-5 SL Benfica *
| 23.02.2010 | SL Benfica | 4-0 | Hertha BSC | |
| 18.02.2010 | Hertha BSC | 1-1 | SL Benfica | |
Everton 2-4 Sporting Lisboa *
| 25.02.2010 | Sporting Lisboa | 3-0 | Everton | |
| 16.02.2010 | Everton | 2-1 | Sporting Lisboa | |
*Liverpool 4-1 Unirea Urziceni
| 25.02.2010 | Unirea Urziceni | 1-3 | Liverpool | |
| 18.02.2010 | Liverpool | 1-0 | Unirea Urziceni | |
Club Brugge 1-3 Valencia CF *
| 25.02.2010 | Valencia CF | 3-0 AET | Club Brugge | |
| 18.02.2010 | Club Brugge | 1-0 | Valencia CF | |
Villarreal CF 3-6 VfL Wolfsburg *
| 25.02.2010 | VfL Wolfsburg | 4-1 | Villarreal CF | |
| 18.02.2010 | Villarreal CF | 2-2 | VfL Wolfsburg | |
FC Twente 2-4 Werder Bremen *
| 25.02.2010 | Werder Bremen | 4-1 | FC Twente | |
| 18.02.2010 | FC Twente | 1-0 | Werder Bremen | |