*Atlético Madrid 2-2 Sporting Lisboa
| 18.03.2010 | Sporting Lisboa | 2-2 | Atlético Madrid | |
| 11.03.2010 | Atlético Madrid | 0-0 | Sporting Lisboa | |
*Hamburger SV 6-5 Anderlecht
| 18.03.2010 | Anderlecht | 4-3 | Hamburger SV | |
| 11.03.2010 | Hamburger SV | 3-1 | Anderlecht | |
Juventus FC 4-5 Fulham *
| 18.03.2010 | Fulham | 4-1 | Juventus FC | |
| 11.03.2010 | Juventus FC | 3-1 | Fulham | |
*SL Benfica 3-2 Olympique Marseille
| 18.03.2010 | Olympique Marseille | 1-2 | SL Benfica | |
| 11.03.2010 | SL Benfica | 1-1 | Olympique Marseille | |
Panathinaikos 1-4 Standard Liège *
| 18.03.2010 | Standard Liège | 1-0 | Panathinaikos | |
| 11.03.2010 | Panathinaikos | 1-3 | Standard Liège | |
Rubin Kazan 2-3 VfL Wolfsburg *
| 18.03.2010 | VfL Wolfsburg | 2-1 AET | Rubin Kazan | |
| 11.03.2010 | Rubin Kazan | 1-1 | VfL Wolfsburg | |
*Valencia CF 5-5 Werder Bremen
| 18.03.2010 | Werder Bremen | 4-4 | Valencia CF | |
| 11.03.2010 | Valencia CF | 1-1 | Werder Bremen | |