*Manchester City 2-2 AaB Aalborg
| 19.03.2009 | AaB Aalborg | 2-0/p.3-4 | Manchester City | |
| 12.03.2009 | Manchester City | 2-0 | AaB Aalborg | |
*Werder Bremen 3-2 AS Saint-Étienne
| 18.03.2009 | AS Saint-Étienne | 2-2 | Werder Bremen | |
| 12.03.2009 | Werder Bremen | 1-0 | AS Saint-Étienne | |
*Hamburger SV 4-3 Galatasaray SK
| 19.03.2009 | Galatasaray SK | 2-3 | Hamburger SV | |
| 12.03.2009 | Hamburger SV | 1-1 | Galatasaray SK | |
*Dynamo Kyiv 3-3 Metalist Kharkiv
| 19.03.2009 | Metalist Kharkiv | 3-2 | Dynamo Kyiv | |
| 12.03.2009 | Dynamo Kyiv | 1-0 | Metalist Kharkiv | |
*Olympique Marseille 4-3 AFC Ajax
| 18.03.2009 | AFC Ajax | 2-2 AET | Olympique Marseille | |
| 12.03.2009 | Olympique Marseille | 2-1 | AFC Ajax | |
*Paris St. Germain 1-0 SC Braga
| 19.03.2009 | SC Braga | 0-1 | Paris St. Germain | |
| 12.03.2009 | Paris St. Germain | 0-0 | SC Braga | |
CSKA Moscow 1-2 Shakhtar Donetsk *
| 19.03.2009 | Shakhtar Donetsk | 2-0 | CSKA Moscow | |
| 12.03.2009 | CSKA Moscow | 1-0 | Shakhtar Donetsk | |
*Udinese Calcio 2-1 Zenit
| 19.03.2009 | Zenit | 1-0 | Udinese Calcio | |
| 12.03.2009 | Udinese Calcio | 2-0 | Zenit | |