Fast Clube/AM 1-5 ABC Natal/RN *
| 20.03.2009 | ABC Natal/RN | 4-0 | Fast Clube/AM | |
| 05.03.2009 | Fast Clube/AM | 1-1 | ABC Natal/RN | |
*Águia de Marabá/PA 3-1 América Mineiro/MG
| 20.03.2009 | América Mineiro/MG | 0-1 | Águia de Marabá/PA | |
| 05.03.2009 | Águia de Marabá/PA | 2-1 | América Mineiro/MG | |
*Confiança/SE 3-2 América/RN
| 19.03.2009 | América/RN | 0-0 | Confiança/SE | |
| 05.03.2009 | Confiança/SE | 3-2 | América/RN | |
Atlético Roraima/RR 1-3 Goiás/GO *
| 05.03.2009 | Atlético Roraima/RR | 1-3 | Goiás/GO | |
Potiguar-M/RN 3-8 Bahia/BA *
| 20.03.2009 | Bahia/BA | 6-1 | Potiguar-M/RN | |
| 05.03.2009 | Potiguar-M/RN | 2-2 | Bahia/BA | |
Cristal/AP 2-5 Brasiliense/DF *
| 19.03.2009 | Brasiliense/DF | 3-1 | Cristal/AP | |
| 05.03.2009 | Cristal/AP | 1-2 | Brasiliense/DF | |
*Misto/MS 2-2 Campinense/PB
| 05.03.2009 | Campinense/PB | 1-1/p.2-3 | Misto/MS | |
| 19.02.2009 | Misto/MS | 1-1 | Campinense/PB | |
*Central/PE 1-1 Ceará SC/CE
| 19.03.2009 | Ceará SC/CE | 1-1 | Central/PE | |
| 05.03.2009 | Central/PE | 0-0 | Ceará SC/CE | |
Corinthians/PR 0-2 Guarani/SP
| 18.02.2009 | Corinthians/PR | 0-2 | Guarani/SP | |
Dom Pedro/DF 0-2 Botafogo/RJ *
| 06.03.2009 | Dom Pedro/DF | 0-2 | Botafogo/RJ | |
Flamengo/PI 1-4 Vasco da Gama/RJ *
| 18.02.2009 | Flamengo/PI | 1-4 | Vasco da Gama/RJ | |
Nacional de Patos//PB 0-4 Fluminense/RJ *
| 05.03.2009 | Fluminense/RJ | 3-0 | Nacional de Patos//PB | |
| 19.02.2009 | Nacional de Patos//PB | 0-1 | Fluminense/RJ | |
Desportiva Capixaba/ES 1-4 Fortaleza/CE *
| 05.03.2009 | Fortaleza/CE | 3-0 | Desportiva Capixaba/ES | |
| 19.02.2009 | Desportiva Capixaba/ES | 1-1 | Fortaleza/CE | |
*Guaratinguetá/SP 3-2 Caxias/RS
| 05.03.2009 | Caxias/RS | 2-1 | Guaratinguetá/SP | |
| 19.02.2009 | Guaratinguetá/SP | 2-0 | Caxias/RS | |
Holanda/AM 1-5 Coritiba/PR *
| 05.03.2009 | Coritiba/PR | 3-0 | Holanda/AM | |
| 19.02.2009 | Holanda/AM | 1-2 | Coritiba/PR | |
*Icasa/CE 2-2 Portuguesa/SP
| 05.03.2009 | Portuguesa/SP | 1-1/p.3-4 | Icasa/CE | |
| 19.02.2009 | Icasa/CE | 1-1 | Portuguesa/SP | |
Itabaiana/SE 0-5 Atlético Mineiro/MG
| 19.02.2009 | Itabaiana/SE | 0-5 | Atlético Mineiro/MG | |
Itumbiara/GO 0-2 Corinthians/SP *
| 05.03.2009 | Itumbiara/GO | 0-2 | Corinthians/SP | |
*Ivinhema/MS 0-5 Flamengo/RJ
| 05.03.2009 | Ivinhema/MS | 0-5 | Flamengo/RJ | |
Atlético Sorocaba/SP 1-2 Juventude/RS *
| 19.03.2009 | Juventude/RS | 2-0 | Atlético Sorocaba/SP | |
| 05.03.2009 | Atlético Sorocaba/SP | 1-0 | Juventude/RS | |
Moto Clube/MA 1-3 Nautico/PE *
| 19.03.2009 | Nautico/PE | 2-0 | Moto Clube/MA | |
| 05.03.2009 | Moto Clube/MA | 1-1 | Nautico/PE | |
Vilhena/RO 2-8 Ponte Preta/SP *
| 19.03.2009 | Ponte Preta/SP | 6-1 | Vilhena/RO | |
| 05.03.2009 | Vilhena/RO | 1-2 | Ponte Preta/SP | |
Rio Branco/AC 1-6 Santos/SP *
| 19.03.2009 | Santos/SP | 4-0 | Rio Branco/AC | |
| 19.02.2009 | Rio Branco/AC | 1-2 | Santos/SP | |
Sampaio Correa/MA 4-4 Figueirense/SC *
| 05.03.2009 | Figueirense/SC | 2-1 | Sampaio Correa/MA | |
| 19.02.2009 | Sampaio Correa/MA | 3-2 | Figueirense/SC | |
*Americano/RJ 6-2 Santa Cruz/PE
| 06.03.2009 | Santa Cruz/PE | 2-4 | Americano/RJ | |
| 19.02.2009 | Americano/RJ | 2-0 | Santa Cruz/PE | |
Tocantins de Palmas/TO 0-3 Atlético/PR *
| 05.03.2009 | Tocantins de Palmas/TO | 0-3 | Atlético/PR | |
Tupi/MG 2-3 Criciúma/SC *
| 05.03.2009 | Criciúma/SC | 3-0 | Tupi/MG | |
| 19.02.2009 | Tupi/MG | 2-0 | Criciúma/SC | |
União Rondonópolis/MT 1-2 Internacional/RS *
| 04.03.2009 | Internacional/RS | 2-0 | União Rondonópolis/MT | |
| 19.02.2009 | União Rondonópolis/MT | 1-0 | Internacional/RS | |
ASA/AL 2-2 Vitória/BA *
| 19.03.2009 | Vitória/BA | 1-1/p.5-4 | ASA/AL | |
| 05.03.2009 | ASA/AL | 1-1 | Vitória/BA | |