*São José/AP 2-1 América/RN
| 11.03.2010 | América/RN | 0-1 | São José/AP | |
| 25.02.2010 | São José/AP | 1-1 | América/RN | |
Araguaia/MT 1-3 Grêmio/RS *
| 10.02.2010 | Araguaia/MT | 1-3 | Grêmio/RS | |
Araguaína/TO 1-3 Guarani/SP *
| 11.03.2010 | Guarani/SP | 2-1 | Araguaína/TO | |
| 25.02.2010 | Araguaína/TO | 0-1 | Guarani/SP | |
*ASA/AL 2-2 Nacional/AM
| 11.03.2010 | Nacional/AM | 2-2 | ASA/AL | |
| 25.02.2010 | ASA/AL | 0-0 | Nacional/AM | |
ASSU/RN 0-3 Atlético/GO *
| 11.02.2010 | ASSU/RN | 0-3 | Atlético/GO | |
Atlético Roraima/RR 0-7 Portuguesa/SP *
| 25.02.2010 | Atlético Roraima/RR | 0-7 | Portuguesa/SP | |
São Raimundo/PA 3-7 Botafogo/RJ *
| 12.03.2010 | Botafogo/RJ | 4-3 | São Raimundo/PA | |
| 11.02.2010 | São Raimundo/PA | 0-3 | Botafogo/RJ | |
Brasília FC/DF 2-4 Sport Recife/PE *
| 25.02.2010 | Brasília FC/DF | 2-4 | Sport Recife/PE | |
*Chapecoense/SC 4-2 Brasiliense/DF
| 11.03.2010 | Brasiliense/DF | 2-1 | Chapecoense/SC | |
| 25.02.2010 | Chapecoense/SC | 3-0 | Brasiliense/DF | |
Cerâmica/RS 2-7 Paraná/PR *
| 25.02.2010 | Paraná/PR | 6-1 | Cerâmica/RS | |
| 10.02.2010 | Cerâmica/RS | 1-1 | Paraná/PR | |
Confiança/SE 1-3 Fluminense/RJ *
| 10.03.2010 | Fluminense/RJ | 2-0 | Confiança/SE | |
| 25.02.2010 | Confiança/SE | 1-1 | Fluminense/RJ | |
*Corinthians/PR 2-0 Juventude/RS
| 25.02.2010 | Juventude/RS | 0-1 | Corinthians/PR | |
| 10.02.2010 | Corinthians/PR | 1-0 | Juventude/RS | |
Luverdense/MT 0-2 Coritiba/PR *
| 11.03.2010 | Coritiba/PR | 1-0 | Luverdense/MT | |
| 25.02.2010 | Luverdense/MT | 0-1 | Coritiba/PR | |
Ituiutaba/MG 2-3 Goiás/GO *
| 11.03.2010 | Goiás/GO | 0-0 | Ituiutaba/MG | |
| 25.02.2010 | Ituiutaba/MG | 2-3 | Goiás/GO | |
Ivinhema/MS 2-4 Nautico/PE *
| 11.03.2010 | Nautico/PE | 3-1 | Ivinhema/MS | |
| 25.02.2010 | Ivinhema/MS | 1-1 | Nautico/PE | |
Juventus/AC 0-7 Atlético Mineiro/MG *
| 25.02.2010 | Juventus/AC | 0-7 | Atlético Mineiro/MG | |
JV Lideral/MA 0-4 Ponte Preta/SP *
| 25.02.2010 | Ponte Preta/SP | 4-0 | JV Lideral/MA | |
| 11.02.2010 | JV Lideral/MA | 0-0 | Ponte Preta/SP | |
Flamengo/PI 0-5 Palmeiras/SP *
| 26.02.2010 | Palmeiras/SP | 4-0 | Flamengo/PI | |
| 11.02.2010 | Flamengo/PI | 0-1 | Palmeiras/SP | |
Picos/PI 0-5 Ceará SC/CE *
| 25.02.2010 | Picos/PI | 0-5 | Ceará SC/CE | |
Potyguar-S/RN 1-3 Paysandu/PA *
| 25.02.2010 | Paysandu/PA | 3-1 | Potyguar-S/RN | |
| 11.02.2010 | Potyguar-S/RN | 0-0 | Paysandu/PA | |
América/AM 0-4 Santa Cruz/PE *
| 11.03.2010 | Santa Cruz/PE | 3-0 | América/AM | |
| 25.02.2010 | América/AM | 0-1 | Santa Cruz/PE | |
Naviraiense/MS 0-11 Santos/SP *
| 11.03.2010 | Santos/SP | 10-0 | Naviraiense/MS | |
| 25.02.2010 | Naviraiense/MS | 0-1 | Santos/SP | |
São Domingos/SE 2-3 Sampaio Correa/MA *
| 26.02.2010 | Sampaio Correa/MA | 2-1 | São Domingos/SE | |
| 11.02.2010 | São Domingos/SE | 1-1 | Sampaio Correa/MA | |
São Mateus/ES 2-6 Remo/PA *
| 11.03.2010 | Remo/PA | 4-1 | São Mateus/ES | |
| 25.02.2010 | São Mateus/ES | 1-2 | Remo/PA | |
Tigres do Brasil/RJ 4-5 Fortaleza/CE *
| 25.02.2010 | Fortaleza/CE | 3-2 | Tigres do Brasil/RJ | |
| 11.02.2010 | Tigres do Brasil/RJ | 2-2 | Fortaleza/CE | |
*Votoraty/SP 5-2 Treze/PB
| 25.02.2010 | Treze/PB | 2-1 | Votoraty/SP | |
| 10.02.2010 | Votoraty/SP | 4-0 | Treze/PB | |
*Uberaba/MG 3-0 Londrina/PR
| 11.03.2010 | Londrina/PR | 0-2 | Uberaba/MG | |
| 25.02.2010 | Uberaba/MG | 1-0 | Londrina/PR | |
Sousa/PB 1-2 Vasco da Gama/RJ *
| 25.02.2010 | Vasco da Gama/RJ | 0-0 | Sousa/PB | |
| 11.02.2010 | Sousa/PB | 1-2 | Vasco da Gama/RJ | |
Vilhena/RO 2-6 Atlético/PR *
| 25.02.2010 | Atlético/PR | 4-0 | Vilhena/RO | |
| 11.02.2010 | Vilhena/RO | 2-2 | Atlético/PR | |
SC Corinthians/AL 3-5 Vitória/BA *
| 11.03.2010 | Vitória/BA | 4-0 | SC Corinthians/AL | |
| 24.02.2010 | SC Corinthians/AL | 3-1 | Vitória/BA | |
Vitória/ES 0-2 Bahia/BA *
| 11.02.2010 | Vitória/ES | 0-2 | Bahia/BA | |
Ypiranga/RS 0-3 Avai FC/SC *
| 25.02.2010 | Ypiranga/RS | 0-3 | Avai FC/SC | |