Goianésia/GO 3-4 ABC Natal/RN *
| 21.04.2016 | ABC Natal/RN | 3-2 | Goianésia/GO | |
| 17.03.2016 | Goianésia/GO | 1-1 | ABC Natal/RN | |
*Aparecidense/GO 4-1 Sport Recife/PE
| 29.04.2016 | Sport Recife/PE | 1-2 | Aparecidense/GO | |
| 07.04.2016 | Aparecidense/GO | 2-0 | Sport Recife/PE | |
*Ypiranga/RS 4-2 Atlético/GO
| 08.04.2016 | Atlético/GO | 0-2 | Ypiranga/RS | |
| 17.03.2016 | Ypiranga/RS | 2-2 | Atlético/GO | |
Operario VG /MT 1-2 Avai FC/SC *
| 29.04.2016 | Avai FC/SC | 2-0 | Operario VG /MT | |
| 22.04.2016 | Operario VG /MT | 1-0 | Avai FC/SC | |
Linense/SP 2-2 Botafogo/PB *
| 07.04.2016 | Botafogo/PB | 1-1/p.6-5 | Linense/SP | |
| 17.03.2016 | Linense/SP | 1-1 | Botafogo/PB | |
Coruripe/AL 1-2 Botafogo/RJ *
| 29.04.2016 | Botafogo/RJ | 1-1 | Coruripe/AL | |
| 06.04.2016 | Coruripe/AL | 0-1 | Botafogo/RJ | |
Brasil Pelotas/RS 1-2 Atlético/PR *
| 14.04.2016 | Atlético/PR | 1-0 | Brasil Pelotas/RS | |
| 18.03.2016 | Brasil Pelotas/RS | 1-1 | Atlético/PR | |
Brasília FC/DF 0-2 Bragantino/SP *
| 20.04.2016 | Brasília FC/DF | 0-2 | Bragantino/SP | |
Caldense/MG 2-3 Ponte Preta/SP *
| 15.04.2016 | Ponte Preta/SP | 1-1 | Caldense/MG | |
| 08.04.2016 | Caldense/MG | 1-2 | Ponte Preta/SP | |
Confiança/SE 1-3 Flamengo/RJ *
| 21.04.2016 | Flamengo/RJ | 3-0 | Confiança/SE | |
| 17.03.2016 | Confiança/SE | 1-0 | Flamengo/RJ | |
*Operário F./PR 3-2 Criciúma/SC
| 29.04.2016 | Criciúma/SC | 1-1 | Operário F./PR | |
| 13.04.2016 | Operário F./PR | 2-1 | Criciúma/SC | |
Campinense/PB 2-3 Cruzeiro/MG *
| 06.05.2016 | Cruzeiro/MG | 3-2 | Campinense/PB | |
| 21.04.2016 | Campinense/PB | 0-0 | Cruzeiro/MG | |
Estanciano/SE 1-3 Paraná/PR *
| 22.04.2016 | Paraná/PR | 2-0 | Estanciano/SE | |
| 07.04.2016 | Estanciano/SE | 1-1 | Paraná/PR | |
Imperatriz/MA 1-3 Fortaleza/CE *
| 29.04.2016 | Fortaleza/CE | 2-0 | Imperatriz/MA | |
| 17.03.2016 | Imperatriz/MA | 1-1 | Fortaleza/CE | |
*Galvez/AC 2-1 Rio Branco/AC
| 28.04.2016 | Rio Branco/AC | 1-1 | Galvez/AC | |
| 21.04.2016 | Galvez/AC | 1-0 | Rio Branco/AC | |
*Gama/DF 3-3 América/RN
| 26.05.2016 | América/RN | 3-2 | Gama/DF | |
| 14.05.2016 | Gama/DF | 1-0 | América/RN | |
Globo FC/RN 1-3 Bahia/BA *
| 07.04.2016 | Bahia/BA | 3-1 | Globo FC/RN | |
| 17.03.2016 | Globo FC/RN | 0-0 | Bahia/BA | |
Guarany/CE 0-3 Coritiba/PR *
| 14.04.2016 | Guarany/CE | 0-3 | Coritiba/PR | |
Inter de Lages/SC 2-2 Sampaio Correa/MA *
| 21.04.2016 | Sampaio Correa/MA | 0-1 | Inter de Lages/SC | |
| 07.04.2016 | Inter de Lages/SC | 1-2 | Sampaio Correa/MA | |
Ivinhema/MS 0-2 CRB/AL *
| 07.04.2016 | Ivinhema/MS | 0-2 | CRB/AL | |
Comercial/MS 1-2 Joinville/SC *
| 05.05.2016 | Joinville/SC | 1-0 | Comercial/MS | |
| 21.04.2016 | Comercial/MS | 1-1 | Joinville/SC | |
*Juazeirense/BA 1-1 Cuiabá/MT
| 28.04.2016 | Cuiabá/MT | 1-0/p.4-5 | Juazeirense/BA | |
| 07.04.2016 | Juazeirense/BA | 1-0 | Cuiabá/MT | |
Lajeadense/RS 0-2 Figueirense/SC *
| 15.04.2016 | Lajeadense/RS | 0-2 | Figueirense/SC | |
Parauapebas/PA 0-7 Londrina/PR *
| 07.04.2016 | Londrina/PR | 6-0 | Parauapebas/PA | |
| 17.03.2016 | Parauapebas/PA | 0-1 | Londrina/PR | |
*Dom Bosco/MT 3-1 Nacional/AM
| 28.04.2016 | Nacional/AM | 1-1 | Dom Bosco/MT | |
| 21.04.2016 | Dom Bosco/MT | 2-0 | Nacional/AM | |
Parnahyba/PI 2-2 Portuguesa/SP *
| 27.04.2016 | Portuguesa/SP | 1-0 | Parnahyba/PI | |
| 14.04.2016 | Parnahyba/PI | 2-1 | Portuguesa/SP | |
Independente/PA 1-4 Paysandu/PA *
| 30.04.2016 | Paysandu/PA | 2-0 | Independente/PA | |
| 28.04.2016 | Independente/PA | 1-2 | Paysandu/PA | |
Princesa/AM 1-4 Chapecoense/SC *
| 15.04.2016 | Chapecoense/SC | 2-0 | Princesa/AM | |
| 06.04.2016 | Princesa/AM | 1-2 | Chapecoense/SC | |
RB Brasil/SP 3-4 América Mineiro/MG *
| 29.04.2016 | América Mineiro/MG | 3-2 | RB Brasil/SP | |
| 21.04.2016 | RB Brasil/SP | 1-1 | América Mineiro/MG | |
Remo/PA 1-3 Vasco da Gama/RJ *
| 28.04.2016 | Vasco da Gama/RJ | 2-1 | Remo/PA | |
| 14.04.2016 | Remo/PA | 0-1 | Vasco da Gama/RJ | |
Resende/RJ 3-3 Ceará SC/CE *
| 27.04.2016 | Ceará SC/CE | 1-1 | Resende/RJ | |
| 07.04.2016 | Resende/RJ | 2-2 | Ceará SC/CE | |
Rio Branco/ES 0-1 Santa Cruz/PE
| 07.04.2016 | Santa Cruz/PE | 0-0 | Rio Branco/ES | |
| 17.03.2016 | Rio Branco/ES | 0-1 | Santa Cruz/PE | |
*Ferroviária/SP 2-1 Salgueiro/PE
| 14.04.2016 | Salgueiro/PE | 1-1 | Ferroviária/SP | |
| 17.03.2016 | Ferroviária/SP | 1-0 | Salgueiro/PE | |
Santos Macapá/AP 1-4 Santos/SP *
| 29.04.2016 | Santos/SP | 3-0 | Santos Macapá/AP | |
| 22.04.2016 | Santos Macapá/AP | 1-1 | Santos/SP | |
Tocantinópolis/TO 1-3 Juventude/RS *
| 29.04.2016 | Juventude/RS | 2-0 | Tocantinópolis/TO | |
| 07.04.2016 | Tocantinópolis/TO | 1-1 | Juventude/RS | |
Tombense FC/MG 0-3 Fluminense/RJ *
| 07.04.2016 | Tombense FC/MG | 0-3 | Fluminense/RJ | |
Náutico/RR 3-6 Vitoria Conquista/BA *
| 28.04.2016 | Vitoria Conquista/BA | 3-1 | Náutico/RR | |
| 14.04.2016 | Náutico/RR | 2-3 | Vitoria Conquista/BA | |
*Vitoria Conquista/BA 1-1 Nautico/PE
| 08.04.2016 | Nautico/PE | 1-1 | Vitoria Conquista/BA | |
| 17.03.2016 | Vitoria Conquista/BA | 0-0 | Nautico/PE | |