*Atlético/CE 1-1 Juazeirense/BA
| 18.09.2021 | Juazeirense/BA | 0-0/p.3-5 | Atlético/CE | |
| 11.09.2021 | Atlético/CE | 1-1 | Juazeirense/BA | |
Brasiliense/DF 0-1 Ferroviária/SP *
| 18.09.2021 | Ferroviária/SP | 1-0 | Brasiliense/DF | |
| 12.09.2021 | Brasiliense/DF | 0-0 | Ferroviária/SP | |
Caldense/MG 2-3 Aparecidense/GO *
| 17.09.2021 | Aparecidense/GO | 3-1 | Caldense/MG | |
| 11.09.2021 | Caldense/MG | 1-0 | Aparecidense/GO | |
*Cianorte/PR 3-0 Cascavel/PR
| 18.09.2021 | Cascavel/PR | 0-3 | Cianorte/PR | |
| 12.09.2021 | Cianorte/PR | 0-0 | Cascavel/PR | |
Galvez/AC 0-2 Guarany/CE *
| 20.09.2021 | Guarany/CE | 2-0 | Galvez/AC | |
| 13.09.2021 | Galvez/AC | 0-0 | Guarany/CE | |
*América/RN 3-1 Itabaiana/SE
| 19.09.2021 | Itabaiana/SE | 0-2 | América/RN | |
| 11.09.2021 | América/RN | 1-1 | Itabaiana/SE | |
Bangu/RJ 2-2 Joinville/SC *
| 18.09.2021 | Joinville/SC | 1-1/p.4-3 | Bangu/RJ | |
| 11.09.2021 | Bangu/RJ | 1-1 | Joinville/SC | |
*Moto Clube/MA 4-1 Castanhal/PA
| 19.09.2021 | Castanhal/PA | 1-2 | Moto Clube/MA | |
| 12.09.2021 | Moto Clube/MA | 2-0 | Castanhal/PA | |
Penarol/AM 1-3 4 de Julho/PI *
| 18.09.2021 | 4 de Julho/PI | 3-0 | Penarol/AM | |
| 12.09.2021 | Penarol/AM | 1-0 | 4 de Julho/PI | |
*Caxias/RS 1-1 Portuguesa/SP
| 18.09.2021 | Portuguesa/SP | 1-0/p.1-4 | Caxias/RS | |
| 12.09.2021 | Caxias/RS | 1-0 | Portuguesa/SP | |
Retrô/PE 3-4 ABC Natal/RN *
| 19.09.2021 | ABC Natal/RN | 3-2 | Retrô/PE | |
| 12.09.2021 | Retrô/PE | 1-1 | ABC Natal/RN | |
*Esportivo/RS 1-1 Santo André/SP
| 18.09.2021 | Santo André/SP | 0-1/p.4-5 | Esportivo/RS | |
| 11.09.2021 | Esportivo/RS | 0-1 | Santo André/SP | |
*Paragominas FC/PA 3-1 São Raimundo/RR
| 19.09.2021 | São Raimundo/RR | 1-2 | Paragominas FC/PA | |
| 12.09.2021 | Paragominas FC/PA | 1-0 | São Raimundo/RR | |
Sergipe/SE 3-3 Campinense/PB *
| 18.09.2021 | Campinense/PB | 1-1/p.4-2 | Sergipe/SE | |
| 12.09.2021 | Sergipe/SE | 2-2 | Campinense/PB | |
*Uberlândia/MG 3-1 Nova Mutum/MT
| 19.09.2021 | Nova Mutum/MT | 0-2 | Uberlândia/MG | |
| 12.09.2021 | Uberlândia/MG | 1-1 | Nova Mutum/MT | |
*União Rondonópolis/MT 3-1 Boa Esporte/MG
| 18.09.2021 | Boa Esporte/MG | 1-1 | União Rondonópolis/MT | |
| 11.09.2021 | União Rondonópolis/MT | 2-0 | Boa Esporte/MG | |