*Le Messager Ngozi 2-1 AS Inter Star
| 25.04.2021 | AS Inter Star | 1-1 | Le Messager Ngozi | |
| 21.04.2021 | Le Messager Ngozi | 1-0 | AS Inter Star | |
*Musongati FC 3-1 Buhumuza FC
| 24.04.2021 | Buhumuza FC | 1-3 | Musongati FC | |
| 21.04.2021 | Musongati FC | 0-0 | Buhumuza FC | |
*Bumamuru FC 2-1 BS Dynamik
| 24.04.2021 | BS Dynamik | 0-0 | Bumamuru FC | |
| 21.04.2021 | Bumamuru FC | 2-1 | BS Dynamik | |
TelaViv FC 2-7 Flambeau du Centre *
| 25.04.2021 | Flambeau du Centre | 6-1 | TelaViv FC | |
| 22.04.2021 | TelaViv FC | 1-1 | Flambeau du Centre | |
*Garage Express 5-4 Lumière FC
| 25.04.2021 | Lumière FC | 3-1 | Garage Express | |
| 22.04.2021 | Garage Express | 4-1 | Lumière FC | |
*Olympique Star 4-2 LLB S4 Africa
| 26.04.2021 | LLB S4 Africa | 1-1 | Olympique Star | |
| 21.04.2021 | Olympique Star | 3-1 | LLB S4 Africa | |
*Royal FC (BDI) 5-2 Bujumbura City
| 26.04.2021 | Bujumbura City | 0-3 | Royal FC (BDI) | |
| 22.04.2021 | Royal FC (BDI) | 2-2 | Bujumbura City | |
Rukinzo FC 1-3 Vital'O FC *
| 24.04.2021 | Vital'O FC | 0-1 | Rukinzo FC | |
| 21.04.2021 | Rukinzo FC | 0-3 | Vital'O FC | |