Bohemians 1905 2-3 Slovan Liberec
| 28.10.2009 | Slovan Liberec | 1-1 | Bohemians 1905 | |
| 07.10.2009 | Bohemians 1905 | 1-2 | Slovan Liberec | |
Sokol Zivanice 4-11 FK Jablonec
| 28.10.2009 | FK Jablonec | 5-1 | Sokol Zivanice | |
| 07.10.2009 | Sokol Zivanice | 3-6 | FK Jablonec | |
Hradec Kralove 2-4 FK Teplice
| 11.11.2009 | FK Teplice | 2-1 | Hradec Kralove | |
| 07.10.2009 | Hradec Kralove | 1-2 | FK Teplice | |
*Pribram 2-0 Banik Ostrava
| 27.10.2009 | Banik Ostrava | 0-0 | Pribram | |
| 07.10.2009 | Pribram | 2-0 | Banik Ostrava | |
Znojmo 1-3 Sigma Olomouc
| 28.10.2009 | Sigma Olomouc | 0-0 | Znojmo | |
| 08.10.2009 | Znojmo | 1-3 | Sigma Olomouc | |
SK Kladno 1-4 Viktoria Plzen
| 13.10.2009 | Viktoria Plzen | 1-1 | SK Kladno | |
| 07.10.2009 | SK Kladno | 0-3 | Viktoria Plzen | |
MFK Karviná 1-2 Slavia Praha
| 25.11.2009 | Slavia Praha | 2-0 | MFK Karviná | |
| 14.11.2009 | MFK Karviná | 1-0 | Slavia Praha | |
Ceske Budejovice 0-6 Sparta Praha
| 12.11.2009 | Sparta Praha | 1-0 | Ceske Budejovice | |
| 13.10.2009 | Ceske Budejovice | 0-5 | Sparta Praha | |