*Rayo Vallecano 4-2 Athletic Bilbao
| 11.11.2009 | Athletic Bilbao | 2-2 | Rayo Vallecano | |
| 28.10.2009 | Rayo Vallecano | 2-0 | Athletic Bilbao | |
Atlético Ciudad 3-9 Sevilla FC *
| 10.11.2009 | Sevilla FC | 5-1 | Atlético Ciudad | |
| 27.10.2009 | Atlético Ciudad | 2-4 | Sevilla FC | |
CD Alcoyano 2-3 Valencia CF *
| 10.11.2009 | Valencia CF | 2-2 | CD Alcoyano | |
| 28.10.2009 | CD Alcoyano | 0-1 | Valencia CF | |
*Celta de Vigo 3-1 CD Tenerife
| 10.11.2009 | CD Tenerife | 0-1 | Celta de Vigo | |
| 28.10.2009 | Celta de Vigo | 2-1 | CD Tenerife | |
Cultural Leonesa 0-7 FC Barcelona *
| 10.11.2009 | FC Barcelona | 5-0 | Cultural Leonesa | |
| 28.10.2009 | Cultural Leonesa | 0-2 | FC Barcelona | |
Marbella FC 0-8 Atlético Madrid *
| 10.11.2009 | Atlético Madrid | 6-0 | Marbella FC | |
| 27.10.2009 | Marbella FC | 0-2 | Atlético Madrid | |
UD Salamanca 2-4 Racing Santander *
| 11.11.2009 | Racing Santander | 4-1 | UD Salamanca | |
| 29.10.2009 | UD Salamanca | 1-0 | Racing Santander | |
*Getafe CF 3-1 RCD Espanyol
| 11.11.2009 | RCD Espanyol | 1-1 | Getafe CF | |
| 28.10.2009 | Getafe CF | 2-0 | RCD Espanyol | |
*AD Alcorcón 4-1 Real Madrid
| 10.11.2009 | Real Madrid | 1-0 | AD Alcorcón | |
| 27.10.2009 | AD Alcorcón | 4-0 | Real Madrid | |
Real Valladolid 2-2 Real Mallorca *
| 11.11.2009 | Real Mallorca | 1-0 | Real Valladolid | |
| 29.10.2009 | Real Valladolid | 2-1 | Real Mallorca | |
Real Murcia 0-1 Deportivo La Coruña *
| 10.11.2009 | Deportivo La Coruña | 0-0 | Real Murcia | |
| 28.10.2009 | Real Murcia | 0-1 | Deportivo La Coruña | |
Real Zaragoza 1-1 Málaga CF *
| 10.11.2009 | Málaga CF | 0-0 | Real Zaragoza | |
| 28.10.2009 | Real Zaragoza | 1-1 | Málaga CF | |
*Recreativo Huelva 2-2 Sporting Gijón
| 10.11.2009 | Sporting Gijón | 1-1/p.2-3 | Recreativo Huelva | |
| 27.10.2009 | Recreativo Huelva | 1-1 | Sporting Gijón | |
Hércules CF 3-1 UD Almería
| 12.11.2009 | UD Almería | 0-1 | Hércules CF | |
| 28.10.2009 | Hércules CF | 2-1 | UD Almería | |
CD Puertollano 1-2 Villarreal CF *
| 10.11.2009 | Villarreal CF | 1-0 | CD Puertollano | |
| 29.10.2009 | CD Puertollano | 1-1 | Villarreal CF | |
Xerez CD 1-3 CA Osasuna *
| 10.11.2009 | CA Osasuna | 1-0 | Xerez CD | |
| 28.10.2009 | Xerez CD | 1-2 | CA Osasuna | |